The population dynamics of Periophthalmus barbarus in the Imo River Estuary, southeast Nigeria, were obtained from a twelve month length composition data ranging 6.0- 14.00cm total length TL (mean = 9.2225cm±0.1425; n=2160) corresponding to 1.67- 26.79g Total weight TW (mean = 8.4717g±0.40196). The growth was exponential. The asymptotic length (L∞) of the Powell-Wetheral plot (L∞ = 13.36cm) was seeded into FiSAT II software to obtain best estimates of von Bertalanffy growth parameters as: L∞ = 19.9cmTL, growth curvature (K) = 1.0 year-1; age-and-length-at-age zero years, t0 = - 0.75; Lo= 10.50 cmTL; longetivity, tmax = 3 years. The estimated growth performance index, ϕ′ = 2.59. Other FiSAT II growth parameters were the amplitude of growth oscillation, C = 0.9 and the Winter Point, WP = 0.6, Rn = 0.368. The computed total mortality, Z = 9.57 year -1, natural mortality, M = 2.04 year-1 and fishing mortality, F was 7.53 year-1. Results indicate the fishery is highly exploited with current exploitation ratio, Ecur = 0.79 > Emax = 0.644 > Eopt = 0.5 which suggests stock over-exploitation; corroborated by Z/K ratio (2.396). The rate of exploitation, μ = 0.787 year-1 = Ecur, 79% of the available stock being fished annually. The length-at-first capture Lc=7.76cm cmTL and Lc/L∞ was 0.39, indicating the fish was yet to complete 61% of growth as at the time of capture at Lc; hence, Lopt = 11.85cmTL, which provides least impact and confers better condition factor on the stock should be adopted.