Fungal endophytes were isolated from surface sterilized leaf segments of five medicinal plants collected form district Mandi, Himachal Pradesh, India. Total 373 fungal strains belonging to 15 fungal genera and 18 species, Aspergillus niger, A. flavus, A. clavatus, A. variecolor, P. chrsogenum, Alternaria alternata, Curvularia lunata, Haplosporium sp., Phoma sp., Nigrospora sp., Collectotrichum sp., Cladosporium sp., Stemphyllum sp., Fusarium sp., Geotrichum sp., Phomopsis sp., Trichoderma sp., Rhizopus sp. and some sterile mycelium were isolated from all the plants. The relative frequency, isolation rate and colonization rates of endophytes were used to study the endophytic diversity. The results showed that highest colonization rate (93.05%) was observed in Adhathoda vasica while it was 91.66% in Ocimum sanctum, 85% in Viola odorata, 82.81% Cannabis sativa and lowest (61.11%) in Withania somenifera. Moreover, about the richness and diversity of the endophytic fungi, highest was obtained from O. sanctum, W. somenifera, C. sativa having 8 species each, while lowest (6&4) from A. vasica and V. Odorata respectively. As the role of endophytic organisms in defensive mechanisms of plants is now well established, the present study is an important step to find new and interesting endophytes among the medicinal plants.